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recombinant ccl24  (MedChemExpress)


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    Structured Review

    MedChemExpress recombinant ccl24
    Recombinant Ccl24, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+ccl24/CCL24%2FEotaxin-2%2C+Mouse/pm41781517-52-17-29
    Average 92 stars, based on 1 article reviews
    recombinant ccl24 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Recombinant:

    Article Title: Peritoneal MSCs-derived exosomes suppress CCL24 synthesis through miR-320d delivery contributing to the improvement of peritoneal dialysis-associated fibrosis.
    Article Snippet: Human PMCs (HPMCs) and rat peritoneal mesothelial cells (RPMCs) were purchased from Wuhan Procell Life Technology Co., Ltd. (Catalog numbers: CPH180 and CP-R157) and cultured in the manufacturer's specialized medium (Procell, CM-H180 and CM-R157, respectively) at 37°C with 5% CO2. .. HPMCs were treated with the supernatant from M2 macrophages with different treatments or together with pre-treatment of recombinant CCL24 (100 ng/mL[18], Gibco, 300- 33-5UG) or CCR3 inhibitor ALK4290 (5μM[19], MCE, HY136788, Shanghai, CN) or p38 inhibitor SB203580 (10μM[20], MCE, HY10256) for 1 h. Cell transfection CCL24 siRNA (SIGS0007801-1), miR-320d inhibitor (miR20006764-1-5), and miR320d mimic (miR10006764-1-5) were obtained from RIBOBIO (Guangzhou, CN). .. Synthetic DNA fragments of KLF7 (Vectorbuilder, VB900007-8978mzp, Guangzhou, CN) were inserted into pRP[Exp]-EGFP/Puro-CAG vector.

    Transfection:

    Article Title: Peritoneal MSCs-derived exosomes suppress CCL24 synthesis through miR-320d delivery contributing to the improvement of peritoneal dialysis-associated fibrosis.
    Article Snippet: Human PMCs (HPMCs) and rat peritoneal mesothelial cells (RPMCs) were purchased from Wuhan Procell Life Technology Co., Ltd. (Catalog numbers: CPH180 and CP-R157) and cultured in the manufacturer's specialized medium (Procell, CM-H180 and CM-R157, respectively) at 37°C with 5% CO2. .. HPMCs were treated with the supernatant from M2 macrophages with different treatments or together with pre-treatment of recombinant CCL24 (100 ng/mL[18], Gibco, 300- 33-5UG) or CCR3 inhibitor ALK4290 (5μM[19], MCE, HY136788, Shanghai, CN) or p38 inhibitor SB203580 (10μM[20], MCE, HY10256) for 1 h. Cell transfection CCL24 siRNA (SIGS0007801-1), miR-320d inhibitor (miR20006764-1-5), and miR320d mimic (miR10006764-1-5) were obtained from RIBOBIO (Guangzhou, CN). .. Synthetic DNA fragments of KLF7 (Vectorbuilder, VB900007-8978mzp, Guangzhou, CN) were inserted into pRP[Exp]-EGFP/Puro-CAG vector.



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    a Stainings of Vim, CD45 and RFP on freshly purified PSCs isolated from NEMO ΔCol1a2.RFP mice (3-week time point). b , c PSCs formed fibroblastoid morphology and express RFP. RFP and nestin stainings on PSCs after 24 h in culture. d Immunoblot of NEMO on PSC protein extracts. TOM20: loading control. WT: PSC WT ; NEMO: PSC ΔNEMO . e , f Gene expressions in PSCs analyzed by qPCR (PSC WT : n = 3; PSC ΔNEMO : n = 4). g Measurement of <t>CCL24</t> level by ELISA from culture media collected at 48 h ( n = 8). Whiskers: Min to Max. T -test (two-tailed): * p < 0.05; ** p < 0.01. h , i Transwell migration assay indicating the fold change of the immune cells towards different media. Media: PSC WT (WT) and PSC ΔNEMO (NEMO) conditioned media (72 h); media with an addition of CCL24 ligand or neutralization with CCL24 antibody (ab). n = 3. One-way ANOVA: * p < 0.05. j Staining of Siglec-F on the transmigrated cells. DAPI: nuclei. Scale bar: 100 µm. All n numbers represent biological replicates.
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    Image Search Results


    a Stainings of Vim, CD45 and RFP on freshly purified PSCs isolated from NEMO ΔCol1a2.RFP mice (3-week time point). b , c PSCs formed fibroblastoid morphology and express RFP. RFP and nestin stainings on PSCs after 24 h in culture. d Immunoblot of NEMO on PSC protein extracts. TOM20: loading control. WT: PSC WT ; NEMO: PSC ΔNEMO . e , f Gene expressions in PSCs analyzed by qPCR (PSC WT : n = 3; PSC ΔNEMO : n = 4). g Measurement of CCL24 level by ELISA from culture media collected at 48 h ( n = 8). Whiskers: Min to Max. T -test (two-tailed): * p < 0.05; ** p < 0.01. h , i Transwell migration assay indicating the fold change of the immune cells towards different media. Media: PSC WT (WT) and PSC ΔNEMO (NEMO) conditioned media (72 h); media with an addition of CCL24 ligand or neutralization with CCL24 antibody (ab). n = 3. One-way ANOVA: * p < 0.05. j Staining of Siglec-F on the transmigrated cells. DAPI: nuclei. Scale bar: 100 µm. All n numbers represent biological replicates.

    Journal: Communications Biology

    Article Title: Functional IKK/NF-κB signaling in pancreatic stellate cells is essential to prevent autoimmune pancreatitis

    doi: 10.1038/s42003-022-03371-3

    Figure Lengend Snippet: a Stainings of Vim, CD45 and RFP on freshly purified PSCs isolated from NEMO ΔCol1a2.RFP mice (3-week time point). b , c PSCs formed fibroblastoid morphology and express RFP. RFP and nestin stainings on PSCs after 24 h in culture. d Immunoblot of NEMO on PSC protein extracts. TOM20: loading control. WT: PSC WT ; NEMO: PSC ΔNEMO . e , f Gene expressions in PSCs analyzed by qPCR (PSC WT : n = 3; PSC ΔNEMO : n = 4). g Measurement of CCL24 level by ELISA from culture media collected at 48 h ( n = 8). Whiskers: Min to Max. T -test (two-tailed): * p < 0.05; ** p < 0.01. h , i Transwell migration assay indicating the fold change of the immune cells towards different media. Media: PSC WT (WT) and PSC ΔNEMO (NEMO) conditioned media (72 h); media with an addition of CCL24 ligand or neutralization with CCL24 antibody (ab). n = 3. One-way ANOVA: * p < 0.05. j Staining of Siglec-F on the transmigrated cells. DAPI: nuclei. Scale bar: 100 µm. All n numbers represent biological replicates.

    Article Snippet: Using a recombinant CCL24 (Peprotech 250-22), we first confirmed that the addition of CCL24 to the IMDM medium could promote a transwell migration (Fig. ).

    Techniques: Purification, Isolation, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Transwell Migration Assay, Neutralization, Staining

    a Stainings of Vim, CD45 and RFP on freshly purified PSCs isolated from NEMO ΔCol1a2.RFP mice (3-week time point). b , c PSCs formed fibroblastoid morphology and express RFP. RFP and nestin stainings on PSCs after 24 h in culture. d Immunoblot of NEMO on PSC protein extracts. TOM20: loading control. WT: PSC WT ; NEMO: PSC ΔNEMO . e , f Gene expressions in PSCs analyzed by qPCR (PSC WT : n = 3; PSC ΔNEMO : n = 4). g Measurement of CCL24 level by ELISA from culture media collected at 48 h ( n = 8). Whiskers: Min to Max. T -test (two-tailed): * p < 0.05; ** p < 0.01. h , i Transwell migration assay indicating the fold change of the immune cells towards different media. Media: PSC WT (WT) and PSC ΔNEMO (NEMO) conditioned media (72 h); media with an addition of CCL24 ligand or neutralization with CCL24 antibody (ab). n = 3. One-way ANOVA: * p < 0.05. j Staining of Siglec-F on the transmigrated cells. DAPI: nuclei. Scale bar: 100 µm. All n numbers represent biological replicates.

    Journal: Communications Biology

    Article Title: Functional IKK/NF-κB signaling in pancreatic stellate cells is essential to prevent autoimmune pancreatitis

    doi: 10.1038/s42003-022-03371-3

    Figure Lengend Snippet: a Stainings of Vim, CD45 and RFP on freshly purified PSCs isolated from NEMO ΔCol1a2.RFP mice (3-week time point). b , c PSCs formed fibroblastoid morphology and express RFP. RFP and nestin stainings on PSCs after 24 h in culture. d Immunoblot of NEMO on PSC protein extracts. TOM20: loading control. WT: PSC WT ; NEMO: PSC ΔNEMO . e , f Gene expressions in PSCs analyzed by qPCR (PSC WT : n = 3; PSC ΔNEMO : n = 4). g Measurement of CCL24 level by ELISA from culture media collected at 48 h ( n = 8). Whiskers: Min to Max. T -test (two-tailed): * p < 0.05; ** p < 0.01. h , i Transwell migration assay indicating the fold change of the immune cells towards different media. Media: PSC WT (WT) and PSC ΔNEMO (NEMO) conditioned media (72 h); media with an addition of CCL24 ligand or neutralization with CCL24 antibody (ab). n = 3. One-way ANOVA: * p < 0.05. j Staining of Siglec-F on the transmigrated cells. DAPI: nuclei. Scale bar: 100 µm. All n numbers represent biological replicates.

    Article Snippet: Preincubate the transwell plate at room temperature for 1 h. List of media prepared: IMDM, IMDM with 200 ng/ml recombinant Murine CCL24 (Peprotech 250-22), IMDM with 0.2 μg/ml CCL24 antibody (R&D MAB528-SP), IMDM with 200 ng/ml recombinant Murine CCL24 and CCL24 antibody, PSC WT conditioned media, PSC ΔNEMO conditioned media and PSC ΔNEMO conditioned media with 0.2 μg CCL24 antibody.

    Techniques: Purification, Isolation, Western Blot, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Transwell Migration Assay, Neutralization, Staining